Class 12th Biology Restriction Enzymes

Restriction Enzymes

Class 12th Biology

Chapter: Restriction Enzymes

Topic: Biotechnology Principle and Processes

Restriction Enzymes

Class 12th Biology

Chapter: Restriction Enzymes

Topic: Restriction Enzymes

Restriction Enzymes

Class 12th Biology

Chapter: Restriction Enzymes

Topic: Competent Host and Process of r-DNA Technology

Restriction Enzymes

Class 12th Biology

Chapter: Restriction Enzymes

Topic: Isolation of DNA

Restriction enzymes, also known as restriction endonucleases, are enzymes that cut DNA at specific sequences called restriction sites. They are widely used in molecular biology for a variety of purposes, including gene cloning, genetic engineering, and DNA sequencing.
Restriction enzymes are produced by bacteria as a defense mechanism against invading viruses or foreign DNA. These enzymes recognize and cut DNA at specific sequences, which are usually palindromic and typically four to six base pairs in length. After cutting the DNA, the enzyme leaves sticky or blunt ends, depending on the type of restriction enzyme

There are three types of restriction enzymes: Type I, Type II, and Type III. Type II restriction enzymes are the most commonly used in molecular biology because they are easy to use and have high specificity. Type II restriction enzymes recognize and cut DNA at specific sequences within or near the restriction site, resulting in blunt or sticky ends. The sticky ends can be used to ligate DNA fragments together or to insert them into a vector for cloning